Overlay histogram showing HeLa cells stained with ab109546 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109546, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
![All lanes : Anti-Nucleophosmin (phospho S125) antibody [EPR1856] (ab109546) at 1/10000 dilutionLane 1 : HeLa cell lysateLane 2 : HeLa cell lysate treated with Alkaline PhosphataseLysates/proteins at 10 µg per lane.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/2618_Nucleophosmin-Primary-antibodies-ab109546-1.jpg)
All lanes : Anti-Nucleophosmin (phospho S125) antibody [EPR1856] (ab109546) at 1/10000 dilutionLane 1 : HeLa cell lysateLane 2 : HeLa cell lysate treated with Alkaline PhosphataseLysates/proteins at 10 µg per lane.
ab109546, at a 1/100 dilution, staining Nucleophosmin in formailn-fixed, paraffin-embedded Human breast tissue.
ab109546, at a 1/100 dilution, staining Nucleophosmin in formalin-fixed, paraffin-embedded Human kidney tissue.