Anti-Nucleophosmin (phospho T199) antibody
| Name | Anti-Nucleophosmin (phospho T199) antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab59353 |
| Prices | $385.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB IHC-P ELISA |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthesized phosphopeptide derived from human Nucleophosmin around the phosphorylation site of threonine 199 (RDTPPA) |
| Description | Rabbit Polyclonal |
| Gene | NPM1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab59353, at a 1/50 dilution, staining human Nucleophosmin in breast carcinoma, using Immunohistochemistry, Paraffin embedded tissue, in the absence (left image) and in the presence of the immunizing peptide (right image).
All lanes : Anti-Nucleophosmin (phospho T199) antibody (ab59353) at 1/500 dilutionLane 1 : Extracts from Jurkat cells with no immunizing peptideLane 2 : Extracts from Jurkat cells with immunizing peptideObserved band size : 33 kDa (why is the actual band size different from the predicted?)
ICC/IF image of ab59353 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59353, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Angiogenic factor signaling regulates centrosome duplication in endothelial cells - Angiogenic factor signaling regulates centrosome duplication in endothelial cells
Taylor SM, Nevis KR, Park HL, Rogers GC, Rogers SL, Cook JG, Bautch VL. Blood. 2010 Oct 21;116(16):3108-17.
Cyclin A is redundant in fibroblasts but essential in hematopoietic and embryonic - Cyclin A is redundant in fibroblasts but essential in hematopoietic and embryonic
Kalaszczynska I, Geng Y, Iino T, Mizuno S, Choi Y, Kondratiuk I, Silver DP, Wolgemuth DJ, Akashi K, Sicinski P. Cell. 2009 Jul 23;138(2):352-65.