Anti-Nucleophosmin (phospho T199) antibody [EP1857Y]
| Name | Anti-Nucleophosmin (phospho T199) antibody [EP1857Y] |
|---|---|
| Supplier | Abcam |
| Catalog | ab81551 |
| Prices | $99.00 |
| Sizes | 10 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | EP1857Y |
| Applications | WB IHC-P ICC/IF ICC/IF IP FC |
| Species Reactivities | Human |
| Antigen | Synthetic phosphopeptide corresponding to residues surrounding Theonine 199 of Human Nucleophosmin |
| Description | Rabbit Monoclonal |
| Gene | NPM1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![All lanes : Anti-Nucleophosmin (phospho T199) antibody [EP1857Y] (ab81551) at 1/2500 dilutionLane 1 : lysate from untreated HeLa cells Lane 2 : lysate from HeLa cells treated with CALysates/proteins at 10 µg per lane.SecondaryHRP labelled Goat anti-Rabbit at 1/2000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/2626_Nucleophosmin-Primary-antibodies-ab81551-1.jpg)
All lanes : Anti-Nucleophosmin (phospho T199) antibody [EP1857Y] (ab81551) at 1/2500 dilutionLane 1 : lysate from untreated HeLa cells Lane 2 : lysate from HeLa cells treated with CALysates/proteins at 10 µg per lane.SecondaryHRP labelled Goat anti-Rabbit at 1/2000 dilution
Immunohistochemistry analysis of paraffin-embedded Human lymphoma tissue, using 1/100 ab81551.
Immunofluorescent staing of HeLa cells using 1/100 ab81551.
ab81551 staining Nucleophosmin (phospho T199) in human HeLa cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with formaldehyde, permeabilized with 0.1% Triton x100 in PBS and blocking with 5% serum was performed for 30 minutes at 230C. Samples were incubated with primary antibody (1/150 in PBS) for 1 hour at 23°C. An Alexa Fluor®488-conjugated goat polyclonal to rabbit IgG was used at dilution at 1/100 as secondary antibody.See Abreview
Overlay histogram showing HeLa cells stained with ab76539 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76539, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Product References
Ki-67 is a PP1-interacting protein that organises the mitotic chromosome - Ki-67 is a PP1-interacting protein that organises the mitotic chromosome
Booth DG, Takagi M, Sanchez-Pulido L, Petfalski E, Vargiu G, Samejima K, Imamoto N, Ponting CP, Tollervey D, Earnshaw WC, Vagnarelli P. Elife. 2014 May 27;3:e01641.
The novel chemical entity YTR107 inhibits recruitment of nucleophosmin to sites - The novel chemical entity YTR107 inhibits recruitment of nucleophosmin to sites
Sekhar KR, Reddy YT, Reddy PN, Crooks PA, Venkateswaran A, McDonald WH, Geng L, Sasi S, Van Der Waal RP, Roti JL, Salleng KJ, Rachakonda G, Freeman ML. Clin Cancer Res. 2011 Oct 15;17(20):6490-9.