Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin carcinoma tissue labelling Nucleostemin with ab70345 at 1/200 (1µg/ml). Detection: DAB.
All lanes : Anti-Nucleostemin antibody (ab70345) at 0.04 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : HeLa whole cell lysate at 5 µgLane 4 : 293T whole cell lysate at 50 µgLane 5 : mouse 3T3 whole cell lysate at 50 µg/ml
Detection of Human Nucleostemin by Western Blot of Immunprecipitate. All lanes: ab70345 at 1µg/ml staining Nucleostemin in HeLa whole cell lysates immunoprecipitated at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane) using the following antibodies: Lane 1: an irrelevant antibody Lane 2: ab70345 Detection: Chemiluminescence with exposure times of 10 seconds