Western blot analysis on immunoprecipitation pellet from 293T cell lysate (lane 1) labeling Nucleotide binding protein like, or 1X PBS (Lane 2: negative control) using ab171741 at 1/10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Immunofluorescent analysis of HepG2 cells labeling Nucleotide binding protein like using ab171741 at 1/50 dilution.
All lanes : Anti-Nucleotide binding protein like antibody [EPR11833] (ab171741) at 1/10000 dilutionLane 1 : Human fetal kidney lysateLane 2 : 293T cell lysateLane 3 : HeLa cell lysateLane 4 : HepG2 cell lysateLysates/proteins at 10 µg per lane.