All lanes : Anti-Nup107 antibody [EPR12241] (ab182559) at 1/20000 dilutionLane 1 : 293 cell lysateLane 2 : K562 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Anti-Nup107 antibody [EPR12241] (ab182559) at 1/20000 dilution + HeLa cell lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded Human clear cell carcinoma of kidney tissue labeling Nup107 with ab182559 at 1/50 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling Nup107 with ab182559 at 1/50 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with hematoxylin.
Immunofluorescent analysis of 4% paraformaldehyde-fixed 293T cells labeling Nup107 with ab182559 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor®555) secondary antibody at 1/200 dilution. Counter stained with DAPI (blue).