![All lanes : Anti-NUP133 antibody [EPR10808(B)] (ab155990) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : Ramos cell lysateLane 3 : SH-SY5Y cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/2973_NUP133-Primary-antibodies-ab155990-1.JPG)
All lanes : Anti-NUP133 antibody [EPR10808(B)] (ab155990) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : Ramos cell lysateLane 3 : SH-SY5Y cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilution

ab155990 staining NUP133 in mokey kidney cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 3% BSA + 0.5% Triton X-100 for 45 minutes at 25°C. Samples were incubated with primary antibody (1/1500 in 3% BSA + 0.5% Triton X-100) for 45 minutes at 25°C. An Alexa Fluor® 647-conjugated donkey anti-rabbit IgG polyclonal (2 µg/ml) was used as the secondary antibody.See Abreview

Immunofluorescent analysis of HeLa cells labeling NUP133 with ab155990 at 1/50 dilution.