![All lanes : Anti-NUP155 antibody [EPR17111] (ab199528) at 1/5000 dilutionLane 1 : Jurkat (Human T cell leukemia cells from peripheral blood) cell lysateLane 2 : HEK293 (Human embryonic kidney) cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/3001_ab199528-244280-ab199528WB1.jpg)
All lanes : Anti-NUP155 antibody [EPR17111] (ab199528) at 1/5000 dilutionLane 1 : Jurkat (Human T cell leukemia cells from peripheral blood) cell lysateLane 2 : HEK293 (Human embryonic kidney) cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
![Anti-NUP155 antibody [EPR17111] (ab199528) at 1/2000 dilution + HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/3002_ab199528-244281-ab199528WB2.jpg)
Anti-NUP155 antibody [EPR17111] (ab199528) at 1/2000 dilution + HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
![Anti-NUP155 antibody [EPR17111] (ab199528) at 1/1000 dilution + Human fetal liver lysate at 10 µgSecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/3003_ab199528-244282-ab199528WB3.jpg)
Anti-NUP155 antibody [EPR17111] (ab199528) at 1/1000 dilution + Human fetal liver lysate at 10 µgSecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling NUP155 with ab199528 at 1/2000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Nucleus membrane and cytoplasm staining on HeLa cell line is observed. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).The negative controls are as follows:--ve control 1: ab199528 at 1/2000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.
Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling NUP155 with ab199528 at 1/250 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.