Immunofluorescence analysis of methanol-fixed HeLa cells, labeling NUP98 using ab179909 at 0.5 µg/ml, followed by Alexa Fluor 488-conjugated anti-mouse lgG (green) at 4 µg/ml. DAPI was used to stain DNA (magenta). Upper and middle panels correspond to black-and-white images while the bottom panel represents merged colored images.
![Anti-NUP98 antibody [21A10] - BSA and Azide free (ab179909) at 0.4 µg/ml + HeLa cell extractSecondaryHRP-labeled anti-mouse IgG at 0.4 µg/mldeveloped using the ECL technique](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/3105_ab179909-209551-ab179911WB11.jpg)
Anti-NUP98 antibody [21A10] - BSA and Azide free (ab179909) at 0.4 µg/ml + HeLa cell extractSecondaryHRP-labeled anti-mouse IgG at 0.4 µg/mldeveloped using the ECL technique
Immunofluorescence analysis of methanol-fixed Tetrahymena thermophila cells, labeling NUP98 using ab179909 at 0.5 µg/ml, followed by Alexa Fluor 488-conjugated anti-mouse lgG (green) at 4 µg/ml. DAPI was used to stain DNA (magenta). Upper and middle panels correspond to black-and-white images while the bottom panel represents merged images. Dotted lines represent the outlines of cells. The open arrow indicates the micronucleus. Insets are magnified images showing the position of the micronucleus.
![All lanes : Anti-NUP98 antibody [21A10] - BSA and Azide free (ab179909) at 2 µg/mlLane 1 : Tetrahymena thermophila cell extractLane 2 : Tetrahymena thermophila cell extractdeveloped using the ECL technique](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/3107_ab179909-209549-ab179911WB21.jpg)
All lanes : Anti-NUP98 antibody [21A10] - BSA and Azide free (ab179909) at 2 µg/mlLane 1 : Tetrahymena thermophila cell extractLane 2 : Tetrahymena thermophila cell extractdeveloped using the ECL technique
Immunofluorescence analysis of formaldehyde-fixed, zymolyase-treated, S. pombe cells, labeling NUP98 using ab179909 at 10 µg/ml, followed by Alexa Fluor 488-conjugated anti-mouse lgG (green). DAPI was used to stain DNA (magenta). Upper and middle panels correspond to black-and-white images while the bottom panel represents merged images. Dotted lines represent the outlines of cells.
![All lanes : Anti-NUP98 antibody [21A10] - BSA and Azide free (ab179909) at 2 µg/mlLane 1 : Cell extract from S. pombe wild typeLane 2 : Cell extract from S. pombe expressing endogenously NUP98 fused to a fluorescence proteindeveloped using the ECL technique](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/3109_ab179909-209547-ab179911WB3.jpg)
All lanes : Anti-NUP98 antibody [21A10] - BSA and Azide free (ab179909) at 2 µg/mlLane 1 : Cell extract from S. pombe wild typeLane 2 : Cell extract from S. pombe expressing endogenously NUP98 fused to a fluorescence proteindeveloped using the ECL technique
Immunofluorescence analysis of formaldehyde-fixed, zymolyase-treated, S. cerevisiae cells, labeling NUP98 using ab179909 at 10 µg/ml, followed by Alexa Fluor 488-conjugated anti-mouse lgG (green). DAPI was used to stain DNA (magenta). Upper and middle panels correspond to black-and-white images while the bottom panel represents merged images. Dotted lines represent the outlines of cells.
![Anti-NUP98 antibody [21A10] - BSA and Azide free (ab179909) at 2 µg/ml + S. cerevisiae cell extractdeveloped using the ECL technique](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/3111_ab179909-209545-ab179911WB4.jpg)
Anti-NUP98 antibody [21A10] - BSA and Azide free (ab179909) at 2 µg/ml + S. cerevisiae cell extractdeveloped using the ECL technique
Summary of the suitability of ab179909 for immunological applications. IF: indirect immunofluorescence staining; WB: Western blotting analysis.