Anti-NUPL2 antibody [EPR16545]

• Supplier: Abcam
• Catalog: ab192609
• Prices: $357.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR16545
• Applications: WB IHC-P ICC/IF ICC/IF IP
• Species Reactivities: Mouse, Rat, Human
• Antigen: Recombinant fragment within Human NUPL2 aa 1-150
• Description: Rabbit Monoclonal
• Gene: NUPL2
• Conjugate: Unconjugated

Product images

Immunofluorescent analysis of HeLa cells (4% Paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized) labeling NUPL2 with ab192609 at 1/100 dilution (6.3μg/mL) followed by Goat anti rabbit IgG (AlexaFluor® 488) (ab150077) secondary at 1/400 dilution and counter-stained with DAPI (blue). Negative control also shown.

Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling NUPL2 with ab192609 at 1/100 dilution (6 μg/ml); followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).

Immunohistochemical analysis of paraffin-embedded Human kidney clear cell carcinoma tissue labeling NUPL2 with ab192609 at 1/100 dilution (6 μg/ml); followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling NUPL2 with ab192609 at 1/100 dilution (6 μg/ml);  followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).

All lanes : Anti-NUPL2 antibody [EPR16545] (ab192609) at 1/1000 dilutionLane 1 : C6 cell lysateLane 2 : RAW 264.7 cell lysateLane 3 : PC12 cell lysateLane 4 : NIH 3T3 cell lysateLane 5 : Mouse brain lysateLane 6 : Mouse heart lysateLane 7 : Mouse kidney lysateLane 8 : Mouse spleen lysateLane 9 : Rat brain lysateLane 10 : Rat heart lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Anti-NUPL2 antibody [EPR16545] (ab192609) at 1/1000 dilution + Human fetal liver lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

All lanes : Anti-NUPL2 antibody [EPR16545] (ab192609) at 1/10000 dilutionLane 1 : 293 cell lysateLane 2 : K562 cell lysateLane 3 : HeLa cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Western blot analysis of immunoprecipitation pellet from K562 cell lysate immunoprecipitated using ab192609 at 1/40 dilution (lane 1) or PBS control (lane 2).Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.