All lanes : Anti-NXF3 antibody (ab154916) at 1/10000 dilutionLane 1 : NT2D1 whole cell lysateLane 2 : IMR32 whole cell lysateLane 3 : U-87 MG whole cell lysateLane 4 : MCF7 whole cell lysateLysates/proteins at 30 µg per lane.
Immunohistochemical analysis of paraffin-embedded Human A549 xenograft tissue labeling NXF3 with ab154916 at 1/100 dilution.
Immunofluorescence analysis of paraformaldehyde-fixed MCF7 cells labeling NXF3 with ab154916 at 1/200 dilution (green). Lower image shows cells co-stained with Hoechst 33342.