Anti-Oct-1 antibody [POU5I097]
| Name | Anti-Oct-1 antibody [POU5I097] |
|---|---|
| Supplier | Abcam |
| Catalog | ab51363 |
| Prices | $385.00 |
| Sizes | 50 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | POU5I097 |
| Applications | FC DB WB ICC/IF ICC/IF |
| Species Reactivities | Human |
| Antigen | Recombinant fragment corresponding to Human Oct-1 |
| Description | Mouse Monoclonal |
| Gene | POU2F1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Ab51363 at 1:10 dilution staining HeLa cells; visualised with AlexaFluor®488 Goat Anti-mouse IgG at 1:200 dilution.
![Anti-Oct-1 antibody [POU5I097] (ab51363) at 1/50 dilution + HEK293 whole cell lysate at 50 µgSecondaryAnti mouse IgG antibody at 1/2500 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/3437_ab51363wb.jpg)
Anti-Oct-1 antibody [POU5I097] (ab51363) at 1/50 dilution + HEK293 whole cell lysate at 50 µgSecondaryAnti mouse IgG antibody at 1/2500 dilution
![Overlay histogram showing Jurkat cells stained with ab51363 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab51363, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1:500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/3438_Oct1-Primary-antibodies-ab51363-1.jpg)
Overlay histogram showing Jurkat cells stained with ab51363 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab51363, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1:500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.