Immunofluorescent analysis of Raji cells labeling Oct-2 with ab179808 at 1:100.
All lanes : Anti-Oct-2 antibody [EPR12482] (ab179808) at 1/1000 dilutionLane 1 : Ramos cell lysateLane 2 : Raji cell lysateLane 3 : Daudi cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution
Immunoprecipitation of Oct-2 from Raji cell lysate using ab179808 at 1:10 for IP (lane 1) or 1X PBS (negative control) (lane 2), and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Flow Cytometrical analysis of permeabilized Daudi cells labeling Oct-2 with ab179808 at 1:100 (red) or a rabbit IgG (negative) (green).