Anti-Oct-2 antibody [EPR542]

• Supplier: Abcam
• Catalog: ab92458
• Prices: $385.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR542
• Applications: WB IHC-P ICC/IF FC
• Species Reactivities: Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Oct-2 aa 150-250
• Description: Rabbit Monoclonal
• Gene: POU2F2
• Conjugate: Unconjugated

Product images

ab92458, at 1:250 staining Oct-2 in Human tonsil paraffin embeeded section by immunohistochemistry.

Anti-Oct-2 antibody [EPR542] (ab92458) at 1/1000 dilution + Daudi lysate at 10 µgSecondaryHRP conjugated goat anti-rabbit antibody at 1/2000 dilution

Overlay histogram showing Raji cells stained with ab92458 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92458, 1:50) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1:500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.