Anti-Oct6 antibody
| Name | Anti-Oct6 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab31766 |
| Prices | $394.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-F ICC/IF ICC/IF IHC-F WB |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Mouse Oct6 |
| Description | Rabbit Polyclonal |
| Gene | POU3F1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
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Lane 1 : MarkerLanes 2 - 4 : Anti-Oct6 antibody (ab31766) at 1 µg/mlLane 1 : As aboveLane 2 : Mouse Brain at 20 µgLane 3 : Brain (Mouse) Tissue Lysate - normal tissue, 0 days old (ab7188) at 20 µgLane 4 : Brain (Rat) Whole Cell Lysate - normal tissue at 20 µgSecondaryLanes 2 - 4 : IR Dye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilutionPerformed under reducing conditions.
ab31766 staining Oct6 in rat brain tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue from 4% PFA perfused animals underwent overnight fixation in 4% paraformaldehyde, cryoprotected in 30% sucrose and cut using cryostat.The primary antibody was diluted, 1/100 (PBS + 0.3% Triton X100) and incubated with sample for 18 hours at 20°C. An Alexa Fluor® 488 conjugated goat polyclonal to rabbit IgG was used at 1/1000 dilution, as secondary.This image is courtesy of an Abreview submitted by Dr Sophie Pezet.See Abreview
ICC/IF image of ab31766 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab31766 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Gbx2 directly restricts Otx2 expression to forebrain and midbrain, competing with - Gbx2 directly restricts Otx2 expression to forebrain and midbrain, competing with
Inoue F, Kurokawa D, Takahashi M, Aizawa S. Mol Cell Biol. 2012 Jul;32(13):2618-27.
Triggering neural differentiation of ES cells by subtype switching of - Triggering neural differentiation of ES cells by subtype switching of
Yasuhara N, Shibazaki N, Tanaka S, Nagai M, Kamikawa Y, Oe S, Asally M, Kamachi Y, Kondoh H, Yoneda Y. Nat Cell Biol. 2007 Jan;9(1):72-9. Epub 2006 Dec 10.