Anti-Olig2 antibody

• Supplier: Abcam
• Catalog: ab81093
• Prices: $390.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF ICC/IF WB IHC-P IHC-F FC
• Species Reactivities: Mouse, Rat, Chicken, Human
• Antigen: A synthetic peptide from the N terminal region of human Olig2, conjugated to an immunogenic carrier protein
• Description: Rabbit Polyclonal
• Gene: OLIG3
• Conjugate: Unconjugated

Product images

Anti-Olig2 antibody (ab81093) at 1/1000 dilution + mouse brain lysate (denatured, reduced)

IHC image of ab81093 staining in human normal cerebral cortex formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab81093, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

ICC/IF image of ab81093 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab81093, 1/200) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Immunohistochemical analysis of chicken embryonic spinal cord, staining Olig2 (green) with ab81093 at 1/500 dilution. Sections were blocked with solution containing 5% normal donkey serum and 0.1% Triton X-100 in PBS for 1 hour. Samples were incubated with primary antibody and an AlexaFluor®488-conjugated anti-rabbit IgG was used as the secondary antibody.

Product References

Epigenome-wide differences in pathology-free regions of multiple - Epigenome-wide differences in pathology-free regions of multiple

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Progressive disorganization of paranodal junctions and compact myelin due to loss - Progressive disorganization of paranodal junctions and compact myelin due to loss

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Myelin debris regulates inflammatory responses in an experimental demyelination - Myelin debris regulates inflammatory responses in an experimental demyelination

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A Prominin-1-Rich Pediatric Glioblastoma: Biologic Behavior Is Determined by - A Prominin-1-Rich Pediatric Glioblastoma: Biologic Behavior Is Determined by

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CXCR2 signaling protects oligodendrocytes and restricts demyelination in a mouse - CXCR2 signaling protects oligodendrocytes and restricts demyelination in a mouse

Hosking MP, Tirotta E, Ransohoff RM, Lane TE. PLoS One. 2010 Jun 28;5(6):e11340.