All lanes : Anti-ONECUT2 antibody [EPR9894] (ab181229) at 1/20000 dilutionLane 1 : Human fetal brain lysateLane 2 : SH-SY5Y cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Flow cytometric analysis of Jurkat cells (2% paraformaldehyde-fixed) labeling ONECUT2 with ab181229 at 1/40 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
Anti-ONECUT2 antibody [EPR9894] (ab181229) at 1/100000 dilution + Jurkat cell lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution