ICC/IF image of ab100923 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab100923, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM
All lanes : Anti-OPCML antibody (ab100923) at 1/500 dilutionLane 1 : Human fetal brain lysateLane 2 : HeLa cell lysate
ab100923, at a 1/100 dilution, staining OPCML in formalin fixed, paraffin embedded Human fetal brain tissue by Immunohistochemistry.