CBP (D6C5) Rabbit mAb
| Name | CBP (D6C5) Rabbit mAb |
|---|---|
| Supplier | Cell Signaling Technology |
| Catalog | 7389 |
| Prices | $246.00 |
| Sizes | 100 µl (10 western blots) |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | D6C5 |
| Applications | WB IP ICC/IF ChIP |
| Species Reactivities | Human, Mouse, Rat, Monkey |
| Antigen | Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to the amino terminus of human CBP protein. |
| Description | Rabbit Monoclonal |
| Gene | CREBBP |
| Supplier Page | Shop |
Product images
Western blot analysis of extracts from various cell lines using CBP (D6C5) Rabbit mAb.
Confocal immunofluorescent analysis of HeLa cells using CBP (D6C5) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 293 cells, treated with Forskolin #3828 (30 μM, 1h) and either 20 μl of CBP (D6C5) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729, using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human ALS2 exon 1 primers, SimpleChIP ® Human NR4A3 Promoter Primers #4829, and SimpleChIP ® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.