TBPL2 Antibody (N-term) (Cat# 102-11423)immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TBPL2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Fluorescent confocal image of Hela cell stained with TBPL2 Antibody (N-term)(Cat# 102-11423).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TBPL2 primary antibody (1:25, 1 h at 37°). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). TBPL2 immunoreactivity is localized to Nucleus and Cytoplasm significantly.
TBPL2 Antibody (N-term) (Cat# 102-11423) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the TBPL2 antibody detected the TBPL2 protein (arrow).