Fluorescent confocal image of U251 cell stained with FOXO6 Antibody (Center)(Cat# 102-11517).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with FOXO6 primary antibody (1:25, 1 h at 37°). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). FOXO6 immunoreactivity is localized to Cytoplasm and Nucleus significantly.
FOXO6 Antibody (Center) (Cat# 102-11517) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the FOXO6 antibody detected the FOXO6 protein (arrow).