Anti-Osteocalcin antibody [OCG4]

• Supplier: Abcam
• Catalog: ab13421
• Prices: $384.00
• Sizes: 50 µg
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone: OCG4
• Applications: ELISA WB IHC-F IHC-P ICC/IF ICC/IF
• Species Reactivities: Sheep, Rabbit, Goat, Chicken, Bovine, Dog, Human, Pig
• Antigen: Full length protein (Cow)
• Description: Mouse Monoclonal
• Gene: BGLAP
• Conjugate: Unconjugated

Product images

ICC/IF image of ab13421 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12421, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM

Product References

Empirical evaluation of bone extraction protocols. - Empirical evaluation of bone extraction protocols.

Cleland TP, Voegele K, Schweitzer MH. PLoS One. 2012;7(2):e31443.

Chemical analyses of fossil bone. - Chemical analyses of fossil bone.

Zheng W, Schweitzer MH. Methods Mol Biol. 2012;915:153-72.

A novel role of the sympatho-adrenergic system in regulating valve calcification. - A novel role of the sympatho-adrenergic system in regulating valve calcification.

Osman L, Chester AH, Sarathchandra P, Latif N, Meng W, Taylor PM, Yacoub MH. Circulation. 2007 Sep 11;116(11 Suppl):I282-7.

Role of human valve interstitial cells in valve calcification and their response - Role of human valve interstitial cells in valve calcification and their response

Osman L, Yacoub MH, Latif N, Amrani M, Chester AH. Circulation. 2006 Jul 4;114(1 Suppl):I547-52.