All lanes : Anti-OTUB2 antibody [11A5] (ab117899) at 1/500 dilutionLane 1 : mock-transfected HEK293T cell lysateLane 2 : OTUB2-transfected HEK293T cell lysateLysates/proteins at 5 µg per lane.
All lanes : Anti-OTUB2 antibody [11A5] (ab117899) at 1/500 dilutionLane 1 : HepG2 cell extractLane 2 : HeLa cell extractLane 3 : HT29 cell extractLane 4 : A549 cell extractLane 5 : COS7 cell extractLane 6 : Jurkat cell extractLane 7 : MDCK cell extractLane 8 : PC12 cell extractLane 9 : MCF7 cell extractLysates/proteins at 35 µg per lane.
HEK293T cells transfected with either OTUB2-expressing vector (Red) or empty vector control plasmid (Blue) were immunostained with 1/100 ab117899, and then analyzed by flow cytometry.