Lanes 1 - 3 : Anti-OTUD1 antibody - C-terminal (ab182511) at 1/500 dilutionLanes 4 - 6 : Anti-OTUD1 antibody - C-terminal (ab182511) at 1/1000 dilutionLane 1 : HCT116 whole cell lysateLane 2 : RPE-1 whole cell lysateLane 3 : 293T whole cell lysateLane 4 : HCT116 whole cell lysateLane 5 : RPE-1 whole cell lysateLane 6 : 293T whole cell lysateLysates/proteins at 50 µg per lane.SecondaryHRP-conjugated donkey anti-rabbit IgG polyclonal at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.