Anti-Oxytocin antibody
| Name | Anti-Oxytocin antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab2078 |
| Prices | $382.00 |
| Sizes | 50 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-F ICC/IF ICC/IF IHC-P RIA |
| Species Reactivities | Mouse, Rat, Sheep, Rabbit, Guinea Pig, Human, Pig |
| Antigen | Oxytocin - Thyroglobulin |
| Description | Rabbit Polyclonal |
| Gene | OXT |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Minipigs were deeply anesthetized with a combination of midazolam and ketamine, prior to transcardial perfusion with phosphate buffered 4% paraformaldehyde (pH 7.4). After perfusion, the brains were removed with special care taken to preserve the optic chiasm and the median eminence. Blocks of tissue containing the hypothalami were dissected, postfixed in the same fixative for 1 day and subsequently cryoprotected in 30% sucrose for 3–4 days, prior to freezing. 10 series of 40-mm thick coronal (6 animals), sagittal (1 animal), and horizontal (1 animal) cryostat sections were collected. Coronal sections for immunohistochemistry were maintained at -18°C as free-floating sections in a cryoprotectant poly-ethylene glycol solution for up to four weeks. Immunohistochemistry was performed using the avidin-biotin method. Accordingly, free-floating sections were first rinsed in Tris-buffered saline (TBS; 0.05 M; pH 7.4) for 15 minutes. Incubations with avidin (0.1%) and biotin (0.01%) were each
ab2078 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab2078 at 1/200 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Salivary peptide tyrosine-tyrosine 3-36 modulates ingestive behavior without - Salivary peptide tyrosine-tyrosine 3-36 modulates ingestive behavior without
Hurtado MD, Sergeyev VG, Acosta A, Spegele M, La Sala M, Waler NJ, Chiriboga-Hurtado J, Currlin SW, Herzog H, Dotson CD, Gorbatyuk OS, Zolotukhin S. J Neurosci. 2013 Nov 20;33(47):18368-80.
Oxytocin and vasopressin involved in restraint water-immersion stress mediated by - Oxytocin and vasopressin involved in restraint water-immersion stress mediated by
Zhao DQ, Ai HB. PLoS One. 2011;6(8):e23362.
Oxytocin is expressed by both intrinsic sensory and secretomotor neurons in the - Oxytocin is expressed by both intrinsic sensory and secretomotor neurons in the
Yu Q, Ji R, Gao X, Fu J, Guo W, Song X, Zhao X, Burnstock G, Shi X, He C, Xiang Z. Cell Tissue Res. 2011 May;344(2):227-37.
The anatomy of the Gottingen minipig hypothalamus. - The anatomy of the Gottingen minipig hypothalamus.
Ettrup KS, Sorensen JC, Bjarkam CR. J Chem Neuroanat. 2010 May;39(3):151-65.