All lanes : Anti-p15 INK4b antibody - C-terminal (ab170851) at 1/100 dilutionLane 1 : Non-transfected 293 cell line lysateLane 2 : p15 INK4-transfected 293 cell line lysateLysates/proteins at 2 µg per lane.
Immunohistochemical analysis in formalin-fixed, paraffin-embedded Human lung tissue, labeling p15 INK4b using ab170851 at a 1/10 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.
Confocal immunofluorescence analysis of Hela cells, labeling p15 INK4b using ab170851 at a 1/10 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclei (blue).
Flow cytometric analysis of 293 cells, labeling p15 INK4b using ab170851 at a 1/10 dilution (right histogram), compared to negative control cells (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.