Anti-p16 ARC antibody [EP1551Y]

• Supplier: Abcam
• Catalog: ab51243
• Prices: $401.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EP1551Y
• Applications: ICC/IF ICC/IF FC IP WB IHC-P
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide corresponding to residues on the N-terminus of human p16 ARC
• Description: Rabbit Monoclonal
• Gene: ARPC5
• Conjugate: Unconjugated

Product images

All lanes : Anti-p16 ARC antibody [EP1551Y] (ab51243) at 1/2000 dilutionLane 1 : Human fetal brainLane 2 : Mouse brainLane 3 : Rat brainLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Ab51243 (1:50) staining human p16 ARC in human uterus adenocarcinoma by immunohistochemistry using paraffin embedded tissue.Ab51243 (1:50) staining human p16 ARC in human uterus adenocarcinoma by immunohistochemistry using paraffin embedded tissue.

Overlay histogram showing HeLa cells stained with ab51243 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab51243, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Product References

Interaction between microRNAs and actin-associated protein Arpc5 regulates - Interaction between microRNAs and actin-associated protein Arpc5 regulates

Chang YF, Lee-Chang JS, Imam JS, Buddavarapu KC, Subaran SS, Sinha-Hikim AP, Gorospe M, Rao MK. Proc Natl Acad Sci U S A. 2012 Apr 10;109(15):5750-5. doi:

Kindlin-3-mediated signaling from multiple integrin classes is required for - Kindlin-3-mediated signaling from multiple integrin classes is required for

Schmidt S, Nakchbandi I, Ruppert R, Kawelke N, Hess MW, Pfaller K, Jurdic P, Fassler R, Moser M. J Cell Biol. 2011 Mar 7;192(5):883-97.

Actin and Arp2/3 localize at the centrosome of interphase cells. - Actin and Arp2/3 localize at the centrosome of interphase cells.

Hubert T, Vandekerckhove J, Gettemans J. Biochem Biophys Res Commun. 2011 Jan 7;404(1):153-8. doi: