Anti-p27 KIP 1 antibody
| Name | Anti-p27 KIP 1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab7961 |
| Prices | $400.00 |
| Sizes | 1 ml |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IP IHC-P WB ELISA ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide: AGSVEQTPKKPGLRRRQT , corresponding to C terminal amino acids 181-198 of Human p27 KIP 1 |
| Blocking Peptide | p27 KIP 1 peptide |
| Description | Rabbit Polyclonal |
| Gene | CDKN1B |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF Image of p27 KIP1 staining in Mouse Cor1 cells with ab7961. The cells were permeabilised with 0.1% Triton. Cells were blocked with 1% BSA for 20 minutes at 21°C and incubated with ab7961 (1/250 dilution) for 2 hours at 21°C.See Abreview
ICC/IF image of ab7961 stained Hek293 cells (ab7902). The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7961, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab7961 staining 927 KIP1 in human tonsillar tissue sections by IHC-P (formaldehyde-fixed paraffin-embedded sections). Tissue samples were fixed with formaldehyde. Antigen retrieval was by heat mediation in citrate buffer (20 minutes at 100°C). Samples were incubated with primary antibody 1/25 (background reducer diluent) for 20 minutes at 25°C. An undiluted HRP polymer Goat anti mouse/rabbit IgG (ab6721) was used as secondary antibody.See Abreview
Immunohistochemistical detection of p27 KIP 1 antibody (ab7961) on formaldehyde-fixed paraffin-embedded mouse cerebellum sections. Antigen retrieval step: Heat mediated. Buffer Used: Citric acid pH6.3. Blocking step: 1% BSA for 10 mins @ 21°C. Primary antibody: 1/250 for 2 hours @ 21°C in TBS/BSA/azide. Secondary antibody: anti rabbit IgG Conjugation: Biotin. An excellent immunostaining pattern that is most evident in nuclei. However, a significant number of nuclei are negative for this Ab and the significance of this is as yet unknown.See Abreview
Standard curve for p27 KIP 1 (Analyte: ab56279); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal to p27 KIP 1 (ab54563) at 1µg/ml and Detector Antibody Rabbit polyclonal to p27 KIP 1 (ab7961) at 0.1µg/ml.
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