Anti-P2Y2 antibody
| Name | Anti-P2Y2 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab10270 |
| Prices | $380.00 |
| Sizes | 50 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB IHC-F |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide: CRRTESTPAGSENTKDIRL , corresponding to amino acids 360-377 of Human P2Y2 |
| Blocking Peptide | P2Y2 peptide |
| Description | Rabbit Polyclonal |
| Gene | P2RY2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab10270 at 1/200 dilution staining P2Y2 in Spinal cord dorsal horn by Immunohistochemistry.
Ab10270 staining P2Y2 in rat DRG by immunohistochemistry using frozen tissue.
ICC/IF image of ab10270 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab10270, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
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Lamarca A, Gella A, Martianez T, Segura M, Figueiro-Silva J, Grijota-Martinez C, Trullas R, Casals N. PLoS One. 2014 Jun 6;9(6):e98998.
Role of ecto-NTPDases on UDP-sensitive P2Y(6) receptor activation during - Role of ecto-NTPDases on UDP-sensitive P2Y(6) receptor activation during
Noronha-Matos JB, Costa MA, Magalhaes-Cardoso MT, Ferreirinha F, Pelletier J, Freitas R, Neves JM, Sevigny J, Correia-de-Sa P. J Cell Physiol. 2012 Jun;227(6):2694-709.
Extracellular ATP acts on P2Y2 purinergic receptors to facilitate HIV-1 - Extracellular ATP acts on P2Y2 purinergic receptors to facilitate HIV-1
Seror C, Melki MT, Subra F, Raza SQ, Bras M, Saidi H, Nardacci R, Voisin L, Paoletti A, Law F, Martins I, Amendola A, Abdul-Sater AA, Ciccosanti F, Delelis O, Niedergang F, Thierry S, Said-Sadier N, Lamaze C, Metivier D, Estaquier J, Fimia GM, Falasca L, Casetti R, Modjtahedi N, Kanellopoulos J, Mouscadet JF, Ojcius DM, Piacentini M, Gougeon ML, Kroemer G, Perfettini JL. J Exp Med. 2011 Aug 29;208(9):1823-34.