All lanes : Anti-P4HA2 antibody (ab70887) at 0.04 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : HeLa whole cell lysate at 5 µgLane 4 : 293T whole cell lysate at 50 µgdeveloped using the ECL technique
HeLa whole cell lysate (at 1 mg for IP, 20% of IP loaded) with ab70887 at 1 µg/ml for WB subsequent to IP at 3 µg/mg lysate. Lane 2: control IgG. Detection: Chemiluminescence with an exposure time of 30 seconds.
All lanes : Anti-P4HA2 antibody (ab70887) at 1 µg/mlLane 1 : Whole cell lysate prepared from 9L cellsLane 2 : Whole cell lysate prepared from PC12 cellsLane 3 : Whole cell lysate prepared from RIE-1 cellsLysates/proteins at 20 µg per lane.SecondaryHRP conjugated pig anti-rabbit polyclonal at 1/5000 dilutiondeveloped using the ECL technique