DC-SIGN (D7F5C) XP ® Rabbit mAb

• Supplier: Cell Signaling Technology
• Catalog: 13193
• Prices: $277.00
• Sizes: 100 µl (10 western blots)
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: D7F5C
• Applications: WB IP ICC/IF FC
• Species Reactivities: Human
• Antigen: Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human DC-SIGN protein.
• Description: Rabbit Monoclonal
• Gene: CD209

Product images

Western blot analysis of extracts from human monocyte-derived dendritic cells using DC-SIGN (D7F5C) XP ® Rabbit mAb.

Western blot analysis of extracts from MUTZ-3 cells, undifferentiated (-) or differentiated (+) into interstitial dendritic cells with Human Granulocyte Macrophage Colony Stimulating Factor (hGM-CSF) #8922 (100 ng/ml), Human Interleukin-4 (hIL-4) #8919 (100 ng/ml), and Human Tumor Necrosis Factor-α (hTNF-α) #8902 (2.5 ng/ml) for 7 d, using DC-SIGN (D7F5C) XP ® (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

Immunoprecipitation of DC-SIGN from MUTZ-3 cell extracts differentiated into interstitial dendritic cells with Human Granulocyte Macrophage Colony Stimulating Factor (hGM-CSF) #8922 (100 ng/ml), Human Interleukin-4 (hIL-4) #8919 (100 ng/ml), and Human Tumor Necrosis Factor-α (hTNF-α) #8902 (2.5 ng/ml) for 7 d, using Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (lane 2) or DC-SIGN (D7F5C) XP ® Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using DC-SIGN (D7F5C) XP ® Rabbit mAb.

Confocal immunofluorescent analysis of MUTZ-3 cells, undifferentiated (negative, left) or differentiated (positive, right) into interstitial dendritic cells with Human Granulocyte Macrophage Colony Stimulating Factor (hGM-CSF) #8922 (100 ng/ml), Human Interleukin-4 (hIL-4) #8919 (100 ng/ml), and Human Tumor Necrosis Factor-α (hTNF-α) #8902 (2.5 ng/ml) for 7 d, using DC-SIGN (D7F5C) XP ® Rabbit mAb (green). Actin filaments were labeled with DyLight® 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Flow cytometric analysis of human monocyte-derived dendritic cells stained with anti-human CD11c PE and either Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (left) or DC-SIGN (D7F5C) XP ® Rabbit mAb (right). Anti-rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 647 Conjugate) #4414 was used as a secondary antibody.