Western blot analysis of extracts from MUTZ-3 cells, undifferentiated (-) or differentiated (+) into interstitial dendritic cells with Human Granulocyte Macrophage Colony Stimulating Factor (hGM-CSF) #8922 (100 ng/mL), Human Interleukin-4 (hIL-4) #8919 (100 ng/mL), and Human Tumor Necrosis Factor-α (hTNF-α) #8902 (2.5 ng/mL) for 8 d, using DC-SIGN Antibody (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Western blot analysis of extracts from human monocyte-derived dendritic cells using DC-SIGN Antibody.
Western blot analysis of extracts from 293T cells, mock transfected (-), transfected with a construct expressing Myc/DDK-tagged full-length human DC-SIGN (hDC-SIGN-Myc/DDK; +), or transfected with a construct expressing full-length human DC-SIGNR (hDC-SIGNR; +), using DC-SIGN Antibody.