Anti-p66 alpha antibody [EPR18252]

• Supplier: Abcam
• Catalog: ab188472
• Prices: $347.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR18252
• Applications: IHC-P ICC/IF ICC/IF WB
• Species Reactivities: Mouse, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human p66 alpha aa 150-250
• Description: Rabbit Monoclonal
• Gene: GATAD2A
• Conjugate: Unconjugated

Product images

All lanes : Anti-p66 alpha antibody [EPR18252] (ab188472) at 1/2000 dilutionLane 1 : K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysateLane 2 : NCCIT (Human pluripotent embryonic carcinoma) whole cell lysateLane 3 : 293 (Human epithelial cells from embryonic kidney) whole cell lysateLane 4 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

All lanes : Anti-p66 alpha antibody [EPR18252] (ab188472) at 1/2000 dilutionLane 1 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysateLane 2 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling p66 alpha with ab188472 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on Human colon is observed. Counter stained with Hematoxylin.Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling p66 alpha with ab188472 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on mouse kidney is observed. Counter stained with Hematoxylin.Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling p66 alpha with ab188472 at 1/2000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on HeLa cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).The negative controls are as follows:--ve control 1: ab188472 at 1/2000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.