
All lanes : Anti-P70 S6 Kinase alpha (phospho T229) antibody (ab59208) at 1/500 dilutionLane 1 : Jurkat cell extractLane 2 : Jurkat cell extract with immunizing phosphopeptide

Immunohistochemical analysis of paraffin embedded human brain tissue using ab59208 at 1/50 dilution. Samples were treated -/+ phosphopeptide.

Anti-P70 S6 Kinase alpha (phospho T229) antibody (ab59208) at 1/500 dilution + Lysates prepared from human HT1080 cell line at 10 µgSecondaryHRP-conjugated donkey polyclonal to rabbit IgG at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-P70 S6 Kinase alpha (phospho T229) antibody (ab59208) at 1/500 dilution + Lysate prepared from mouse NIH 3T3 cells at 10 µgSecondaryHRP-conjugated donkey polyclonal to rabbit IgG at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.