Anti-P70 S6 Kinase alpha antibody [EPR13429]

• Supplier: Abcam
• Catalog: ab186753
• Prices: $384.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR13429
• Applications: WB IHC-P ICC/IF ICC/IF FC
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human P70 S6 Kinase alpha aa 350-450 (phospho T390)
• Description: Rabbit Monoclonal
• Gene: RPS6KB1
• Conjugate: Unconjugated

Product images

All lanes : Anti-P70 S6 Kinase alpha antibody [EPR13429] (ab186753) at 1/1000 dilutionLane 1 : 293 cell lysateLane 2 : HeLa cell lysateLane 3 : HepG2 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

All lanes : Anti-P70 S6 Kinase alpha antibody [EPR13429] (ab186753) at 1/1000 dilutionLane 1 : C6 cell lysateLane 2 : Raw264.7 cell lysateLane 3 : PC12 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling P70 S6 Kinase alpha with ab186753 at 1/50 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling P70 S6 Kinase alpha with ab186753 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).

Immunofluorescent analysis of 4% paraformaldehyde-fixed HepG2 cells labeling P70 S6 Kinase alpha with ab186753 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).

Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling P70 S6 Kinase alpha with ab186753 at 1/500 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.