Anti-p95 NBS1 (phospho S432) antibody

• Supplier: Abcam
• Catalog: ab12297
• Prices: $376.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: WB
• Species Reactivities: Human
• Antigen: Synthetic peptide conjugated to KLH derived from within residues 400 - 500 of Human p95 NBS1, phosphorylated at S432
• Description: Rabbit Polyclonal
• Gene: NBN
• Conjugate: Unconjugated

Product images

Dot blot using ab12297 on phospho and non-phospho peptides corresponding to the immunising peptide sequence.Dots 1: 1ng of peptideDots 2: 10ng of peptideDots 3: 100ng of peptide

Lanes 1 - 2 : Anti-p95 NBS1 (phospho S432) antibody (ab12297)Lanes 3 - 4 : NBS1 antibody (recognizes modified and unmodified NBS1)Lane 1 : IP'd lysate from cells transfected with wild type (WT) NBS1.Lane 2 : IP'd lysate from cells transfected with mutant (S432A) NBS1.Lane 3 : IP'd lysate from cells transfected with wild type (WT) NBS1.Lane 4 : IP'd lysate from cells transfected with mutant (S432A) NBS1.Performed under reducing conditions.

All lanes : Anti-p95 NBS1 (phospho S432) antibody (ab12297)Lane 1 : ILB1 cells transfected with empty vector.Lane 2 : ILB1 cells transfected with wild type NBS1.Lane 3 : ILB1 cells transfected with mutant (S432A) NBS1.Performed under reducing conditions.

Anti-p95 NBS1 (phospho S432) antibody (ab12297) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µgSecondaryAnti-Rabbit IgG VHH Single Domain Antibody (HRP) (ab191866) at 1/50000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.