Anti-p95 NBS1 antibody
| Name | Anti-p95 NBS1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab23996 |
| Prices | $378.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human, Chicken, Dog |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 700 to the C-terminus of Human p95 NBS1 |
| Description | Rabbit Polyclonal |
| Gene | NBN |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-p95 NBS1 antibody (ab23996) at 1/5000 dilutionLane 1 : Lysate from cells engineered to be NBS1 defective (SV40 transformed, immortal fibroblasts)Lane 2 : MRC5 cell lysate (SV40 transformed immortal (WT) fibroblasts)Lysates/proteins at 20 µg per lane.Performed under reducing conditions.
All lanes : Anti-p95 NBS1 antibody (ab23996) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell LysateLane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell LysateLane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ICC/IF image of ab23996 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab23996, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) HepG2 and MCF7 cells at 1µg/ml.
Product References
DIM (3,3'-diindolylmethane) confers protection against ionizing radiation by a - DIM (3,3'-diindolylmethane) confers protection against ionizing radiation by a
Fan S, Meng Q, Xu J, Jiao Y, Zhao L, Zhang X, Sarkar FH, Brown ML, Dritschilo A, Rosen EM. Proc Natl Acad Sci U S A. 2013 Nov 12;110(46):18650-5. doi:
MCL-1 localizes to sites of DNA damage and regulates DNA damage response. - MCL-1 localizes to sites of DNA damage and regulates DNA damage response.
Jamil S, Stoica C, Hackett TL, Duronio V. Cell Cycle. 2010 Jul 15;9(14):2843-55. Epub 2010 Jul 11.