Immunocytochemistry/Immunofluorescence analysis of U2OS cells labelling p95 NBS1 with ab175800 at 1:1000. Cells were fixed with PFA and permeabilized with methanol for 2 minutes. Cells were incubated with the primary anitbody overnight at room temperature. Left - p95 NBS1, Middle - DAPI, Right - Merge.
Immunohistochemistry (formalin/PFA-fixed paraffin-embedded) analysis of Human linfoid tissue labelling p95 NBS1 with ab175800 at 1:1000. Antigen retrieval was by microwave boiling for 7 minutes in 10mM TrisHCl, pH9. Left - 4X, Right - 20X.
Immunoprecipitation analysis of U2OS cells labelling p95 NBS1 with ab175800. Protein A beads were crosslinked to the serum.
All lanes : Anti-p95 NBS1 antibody (ab175800)Lane 1 : Control siLane 2 : Nbs1 siLane 3 : NIH 3T3 cell lysate at 40 µg