Anti-PACAP antibody - C-terminal (ab174982) at 1/100 dilution + 293 cell lysate at 35 µg
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human testis tissue labeling PACAP with ab174982 at 1/10 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.
Immunofluorescent analysis of 293 cells labeling PACAP with ab174982 at 1/10 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclei (blue).
Flow cytometric analysis of 293 cells labeling PACAP with ab174982 at 1/10 dilution (right histogram) compared to negative control cells (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.