Anti-PADI1 / PAD1 antibody
| Name | Anti-PADI1 / PAD1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab24008 |
| Prices | $370.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ICC/IF ICC/IF |
| Species Reactivities | Human |
| Antigen | A 15 residue synthetic peptide derived from a domain specific for Human peptidylarginine deiminase type 1 protein |
| Description | Rabbit Polyclonal |
| Gene | PADI1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab24008 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab24008 at 1/1000 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Anti-PADI1 / PAD1 antibody (ab24008) + Human epidermis