Anti-PADI2 / PAD2 antibody

• Supplier: Abcam
• Catalog: ab16478
• Prices: $400.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: FC ICC/IF ICC/IF IHC-P ELISA WB
• Species Reactivities: Rat, Human
• Antigen: Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human PADI2/ PAD2
• Blocking Peptide: Human PADI2 / PAD2 peptide
• Description: Rabbit Polyclonal
• Gene: PADI2
• Conjugate: Unconjugated

Product images

Image courtesy of Human Protein Atlas ab16478 staining PADI2 in female rectum, showing a distinct and strong staining pattern in glandular cells. Paraffin embedded human rectal tissue was incubated with ab16478 (1/100 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. ab16478 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org.

ICC/IF image of ab16478 stained human Hek293 cells. The cells were PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab16478, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

ab16478 staining human peripheral blood mononuclear cells (cultured with M-CSF) by Flow Cytometery. Cells were treated with flow cytometery staining buffer (PBS 0.1% sodium azide 1% BSA) and gating was done on myeloid cells. The primary antibody was diluted 1/10 (PBS 0.1% sodium azide 1% BSA) and incubated with sample for 20 minutes at 25°C. An Alexa Fluor® conjugated goat polyclonal to rabbit IgG was used undiluted as secondary.See Abreview

Anti-PADI2 / PAD2 antibody (ab16478) at 1 µg/ml + Kidney (Human) Tissue Lysate - adult normal tissue (ab30203) at 20 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Product References

PADI2 is significantly associated with rheumatoid arthritis. - PADI2 is significantly associated with rheumatoid arthritis.

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Peptidylarginine deiminases present in the airways during tobacco smoking and - Peptidylarginine deiminases present in the airways during tobacco smoking and

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Peptidylarginine deiminase type 2 is over expressed in the glaucomatous optic - Peptidylarginine deiminase type 2 is over expressed in the glaucomatous optic

Cafaro TA, Santo S, Robles LA, Crim N, Urrets-Zavalia JA, Serra HM. Mol Vis. 2010 Aug 17;16:1654-8.

Mechanical stretching elevates peptidyl arginine deiminase 2 expression in - Mechanical stretching elevates peptidyl arginine deiminase 2 expression in

Algeciras ME, Takahara H, Bhattacharya SK. Curr Eye Res. 2008 Nov;33(11):994-1001.

Synovial fluid is a site of citrullination of autoantigens in inflammatory - Synovial fluid is a site of citrullination of autoantigens in inflammatory

Kinloch A, Lundberg K, Wait R, Wegner N, Lim NH, Zendman AJ, Saxne T, Malmstrom V, Venables PJ. Arthritis Rheum. 2008 Aug;58(8):2287-95.