All lanes : Anti-PADI6 antibody (ab16480) at 1 µg/mlLane 1 : Human ovary lysate Lane 2 : Human testes lysate Lane 3 : Human ovary lysate with Human PADI6 peptide (ab17094) at 1 µg/mlLane 4 : Human testes lysate with Human PADI6 peptide (ab17094) at 1 µg/mlLysates/proteins at 20 µg per lane.SecondaryAlexa Fluor Goat polyclonal to Rabbit IgG at 1/10000 dilutiondeveloped using the ECL technique
ICC/IF image of ab16480 stained HeLa cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab16480, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HepG2 100% methanol fixed cells at 5ug/ml. However, this Fast-Track antibody is not yet fully characterised. This image represents inconclusive preliminary data.