Anti-PAF Receptor antibody
| Name | Anti-PAF Receptor antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab104162 |
| Prices | $370.00 |
| Sizes | 250 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ICC/IF FC |
| Species Reactivities | Mouse, Rat, Human, Pig, Monkey |
| Antigen | Synthetic peptide: MEPHDSSHMDSEFRYTL conjugated to KLH, corresponding to N terminal amino acids 1-17 of Human PAF Receptor (Peptide available as ab107706 |
| Blocking Peptide | PAF Receptor peptide |
| Description | Rabbit Polyclonal |
| Gene | PTAFR |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-PAF Receptor antibody (ab104162) at 1 µg/mlLane 1 : Molecular weight markersLane 2 : A549 membrane fraction at 30 µgSecondaryAnti-rabbit IgG (HRP) conjugate at 1/10000 dilutiondeveloped using the ECL technique
![PAF Receptor was immunoprecipitated using 0.5mg PANC1 whole cell extract, 5µg of Rabbit polyclonal to PAF Receptor and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).The antibody was incubated under agitation with Protein G beads for 10min, PANC1 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab104162.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 39kDa; PAF Receptor](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/6486_ab104162-234722-IPVI005ab10416220m.jpg)
PAF Receptor was immunoprecipitated using 0.5mg PANC1 whole cell extract, 5µg of Rabbit polyclonal to PAF Receptor and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).The antibody was incubated under agitation with Protein G beads for 10min, PANC1 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab104162.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 39kDa; PAF Receptor
Product References
The expression of platelet-activating factor receptor modulates the cisplatin - The expression of platelet-activating factor receptor modulates the cisplatin
Yu Y, Zhang X, Hong S, Zhang M, Cai Q, Zhang M, Jiang W, Xu C. Br J Cancer. 2014 Jul 29;111(3):515-24.
Enhanced permeability responses to inflammation in streptozotocin-induced - Enhanced permeability responses to inflammation in streptozotocin-induced
Yuan D, Xu S, He P. Am J Physiol Heart Circ Physiol. 2014 Jul 1;307(1):H44-53. doi: