Anti-PAI1 antibody

• Supplier: Abcam
• Catalog: ab7205
• Prices: $382.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF ICC/IF ICC/IF WB
• Species Reactivities: Mouse, Rat
• Antigen: Recombinant full length protein (Rat)
• Description: Rabbit Polyclonal
• Gene: Serpine1
• Conjugate: Unconjugated

Product images

Anti-PAI1 antibody (ab7205) at 1/1000 dilution

ICC/IF image of ab7205 stained MEF1 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7205, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Product References

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Stefos GC, Soppa U, Dierssen M, Becker W. PLoS One. 2013 Jun 25;8(6):e67470.

N-terminal tyrosine phosphorylation of caveolin-2 negates anti-proliferative - N-terminal tyrosine phosphorylation of caveolin-2 negates anti-proliferative

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Vitamin D reduces the expression of collagen and key profibrotic factors by - Vitamin D reduces the expression of collagen and key profibrotic factors by

Artaza JN, Norris KC. J Endocrinol. 2009 Feb;200(2):207-21.

Myostatin promotes a fibrotic phenotypic switch in multipotent C3H 10T1/2 cells - Myostatin promotes a fibrotic phenotypic switch in multipotent C3H 10T1/2 cells

Artaza JN, Singh R, Ferrini MG, Braga M, Tsao J, Gonzalez-Cadavid NF. J Endocrinol. 2008 Feb;196(2):235-49.