Anti-PAICS antibody [5B6]
| Name | Anti-PAICS antibody [5B6] |
|---|---|
| Supplier | Abcam |
| Catalog | ab117931 |
| Prices | $384.00 |
| Sizes | 100 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2b |
| Clone | 5B6 |
| Applications | WB ICC/IF ICC/IF FC |
| Species Reactivities | Human |
| Antigen | Recombinant full length Human PAICS produced in HEK293T cells (NP_006443) |
| Description | Mouse Monoclonal |
| Gene | PAICS |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Overlay histogram showing Jurkat cells stained with ab117931 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab117931, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/6631_ab117931-3-ab117931FC.jpg)
Overlay histogram showing Jurkat cells stained with ab117931 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab117931, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
![All lanes : Anti-PAICS antibody [5B6] (ab117931) at 1/2000 dilutionLane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control cDNA Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY PAICS cDNA Lysates/proteins at 5 µg per lane.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/6632_PAICS-Primary-antibodies-ab117931-1.jpg)
All lanes : Anti-PAICS antibody [5B6] (ab117931) at 1/2000 dilutionLane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control cDNA Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY PAICS cDNA Lysates/proteins at 5 µg per lane.
ab117931 at 1/100 dilution staining PAICS in COS7 cells transiently transfected by pCMV6-ENTRY PAICS by Immunofluorescence.