All lanes : Anti-PAM16 antibody [EPR15214] (ab184157) at 1/20000 dilutionLane 1 : HepG2 cell lysateLane 2 : LnCaP cell lysateLane 3 : Jurkat cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Anti-PAM16 antibody [EPR15214] (ab184157) at 1/20000 dilution + Human skeletal muscle lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling PAM16 with ab184157 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained using hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue labeling PAM16 with ab184157 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained using hematoxylin.
Immunofluorescent analysis of 4% paraformaldehyde-fixed HepG2 cells labeling PAM16 with ab184157 at 1/250 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody at 1/200 dilution. Counter stained with DAPI (blue).