Anti-pan Arrestin antibody

• Supplier: Abcam
• Catalog: ab2914
• Prices: $380.00
• Sizes: 200 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF ICC/IF WB IHC-P IP
• Species Reactivities: Rat, Bovine, Human, Mouse, Rabbit, Monkey, Rainbow trout
• Antigen: Synthetic peptide corresponding to Human pan Arrestin aa 384-397
• Description: Rabbit Polyclonal
• Gene: ARRB1
• Conjugate: Unconjugated

Product images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of pan Arrestin showing staining in the cytoplasm and nucleus of paraffin-treated human cerebellum tissue (right) compared with a negative control in the absence of primary antibody (left). Antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then incubated with ab2914 diluted by 3% BSA-PBS at a dilution of 1:500 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of pan Arrestin showing staining in the cytoplasm and nucleus of paraffin-treated rat brain tissue (right) compared with a negative control in the absence of primary antibody (left). Antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then incubated with ab2914 diluted by 3% BSA-PBS at a dilution of 1:500 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of pan Arrestin showing staining in the cytoplasm and nucleus of paraffin-treated rat spleen tissue (right) compared with a negative control in the absence of primary antibody (left). Antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then incubated with ab2914 diluted by 3% BSA-PBS at a dilution of 1:500 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

All lanes : Anti-pan Arrestin antibody (ab2914) at 1/1000 dilutionLane 1 : C6 cell lysateLane 2 : Rat brain cell lysateLane 3 : HeLa cell lysateLysates/proteins at 25 µg per lane.

 ab2914 staining human HEK 293 cells by ICC/IF. The cells were fixed in 4% PFA (in PBS) for 30min. After washing the cells were permeabilised with 0.01% Triton X-100 and then incubated with the primary antibody in PBS-T for 18h at 4C. An Alexa Fluor ® 488 conjugated donkey polyclonal antibody was used as the secondary (staining shown in green). After three 5min washes with PBS the cover slips were mounted with slow fade mounting solution containing DAPI (blue) .See Abreview

Anti-pan Arrestin antibody (ab2914) at 1 µg/ml + Brain (Human) Tissue Lysate - adult normal tissue (ab29466) at 10 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Observed band size : 49 +51 kDa (why is the actual band size different from the predicted?)Additional bands at : 27 kDa. We are unsure as to the identity of these extra bands.Exposure time : 90 secondspan Arrestin contains a number of potential phosphorylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.

Product References

Neurotensin-induced proinflammatory signaling in human colonocytes is regulated - Neurotensin-induced proinflammatory signaling in human colonocytes is regulated

Law IK, Murphy JE, Bakirtzi K, Bunnett NW, Pothoulakis C. J Biol Chem. 2012 Apr 27;287(18):15066-75.

Beta-arrestin 2 is required for B1 receptor-dependent post-translational - Beta-arrestin 2 is required for B1 receptor-dependent post-translational

Kuhr FK, Zhang Y, Brovkovych V, Skidgel RA. FASEB J. 2010 Jul;24(7):2475-83.

The 27-kDa heat shock protein confers cytoprotective effects through a beta - The 27-kDa heat shock protein confers cytoprotective effects through a beta

Rojanathammanee L, Harmon EB, Grisanti LA, Govitrapong P, Ebadi M, Grove BD, Miyagi M, Porter JE. Mol Pharmacol. 2009 Apr;75(4):855-65.

Intracellular mechanisms regulating corticotropin-releasing hormone - Intracellular mechanisms regulating corticotropin-releasing hormone

Markovic D, Punn A, Lehnert H, Grammatopoulos DK. Mol Endocrinol. 2008 Mar;22(3):689-706. Epub 2007 Nov 29.

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Jones BW, Hinkle PM. Mol Pharmacol. 2008 Jul;74(1):195-202.

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Molla-Herman A, Boularan C, Ghossoub R, Scott MG, Burtey A, Zarka M, Saunier S, Concordet JP, Marullo S, Benmerah A. PLoS One. 2008;3(11):e3728.

Platelet-activating factor-induced clathrin-mediated endocytosis requires - Platelet-activating factor-induced clathrin-mediated endocytosis requires

McLaughlin NJ, Banerjee A, Kelher MR, Gamboni-Robertson F, Hamiel C, Sheppard FR, Moore EE, Silliman CC. J Immunol. 2006 Jun 1;176(11):7039-50.