All lanes : Anti-Papillomavirus Regulatory Factor 1 / HDBP2 antibody (ab75727) at 1/100 dilutionLane 1 : Human renal cancer whole cell lysateLane 2 : Human renal cancer whole cell lysateLysates/proteins at 50 µg per lane.SecondaryDonkey Anti-Rabbit IgG H&L (Alexa Fluor® 680) preadsorbed (ab186692)Performed under reducing conditions.
Anti-Papillomavirus Regulatory Factor 1 / HDBP2 antibody (ab75727) at 1/60 dilution + Y79 cell lysate at 35 µg
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue labelled with ab75727 at 1/50 dilution, followed by a peroxidase conjugated secondary antibody and DAB staining.