All lanes : Anti-PAQR5 antibody (ab79517) at 1/500 dilutionLane 1 : Extracts from HUVEC cellsLane 2 : Extracts from HUVEC cells with mmunizing peptide at 10 µgLysates/proteins at 25 µg per lane.
All lanes : Anti-PAQR5 antibody (ab79517) at 500 µgLane 1 : HUVEC cell lysate (positive control)Lane 2 : Non-pregnant ovine caruncle tissue lysateLane 3 : Non-pregnant ovine caruncle tissue lysateLane 4 : Non-pregnant ovine caruncle tissue lysateLane 5 : Day 30 of gestation ovine caruncle tissue lysateLane 6 : Day 30 of gestation ovine caruncle tissue lysateLane 7 : Day 30 of gestation ovine caruncle tissue lysateLysates/proteins at 40 µg per lane.SecondaryHRP-conjugated goat anti-rabbit IgG monoclonal at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ab79517 staining PAQR5 in murine ovary tissue by Immunohistochemistry (PFA-fixed).Tissue was fixed in paraformaldehyde and blocked using 10% normal goat serum for 1 hour at 25°C, then incubated with ab79517 at a 1/500 dilution for 12 hours at 4°C. The secondary used was a biotin conjugated goat anti-rabbit polyclonal used at a 1/500 dilution.ab79517 staining PAQR5 in murine ovary tissue by Immunohistochemistry (PFA-fixed).Tissue was fixed in paraformaldehyde and blocked using 10% normal goat serum for 1 hour at 25°C, then incubated with ab79517 at a 1/500 dilution for 12 hours at 4°C. The secondary used was a biotin conjugated goat anti-rabbit polyclonal used at a 1/500 dilution.See Abreview