Immunofluorescent analysis of 4% paraformaldehyde-fixed MCF7 cells labeling PAR2 with ab180953 at 1/100 dilution followed by Goat anti rabbit IgG (Dylight 488) at 1/200 dilution. Counter stained with Dapi.
Immunohistochemical analysis of paraffin-embedded Human hepatocellualr carcinoma tissue labeling PAR2 with ab180953 at 1/100 dilution, followed by prediluted ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling PAR2 with ab180953 at 1/100 dilution, followed by prediluted ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
All lanes : Anti-PAR2 antibody [EPR13675] (ab180953) at 1/10000 dilutionLane 1 : Fetal liver lysateLane 2 : HepG2 cell lysateLane 3 : MCF-7 cell lysateLane 4 : K562 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution